Agilent Technologies E5500 Series Uživatelská příručka stáhnout pdf (Strana 67)

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixD Library Construction Oligonucleotide Sequences

Barcoded adaptor sequences

Barcode-T-040, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAAAGGGTTACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-041, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTTGTGGGATTGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-042, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGAATGTACTACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-043, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTCGCTAGGGTTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-044, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGGATGATCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-045, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTACTTGGCTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-046, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGGTCGTCGAACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-047, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGAGGGATGGCCTGCTGTACGGCCAAGGCGT-3

′

Barcode-T-048, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCCGTAAGTGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-049, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTATGTCATAAGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-050, 50 µM

5′-CGCCTTGGCCGTACAGCAG3′

5′-CTGCCCCGGGTTCCTCATTCTCTGAAGGCTTGCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-051, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGCAGGAGTCTGCTGTACGGCCAAGGCGT-3′

Barcoded adaptor sequence Length (nt)

1 2 ... 62 63 64 65 66 67 68 69 70 71 72 ... 86 87

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