Agilent Technologies E5500 Series Uživatelská příručka stáhnout pdf (Strana 69)

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixD Library Construction Oligonucleotide Sequences

Barcoded adaptor sequences

Barcode-T-064, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTGCTACACCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-065, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGGGATCAAGCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-066, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGATGTAATGTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-067, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTCCTTAGGGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-068, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCATTGACGACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-069, 50 µM

5′-CGCCTTGGCCGTACAGCAG3′

5′-CTGCCCCGGGTTCCTCATTCTCTGATATGCTTTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-070, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCCCTACAGACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-071, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTACAGGGAACGCTGCTGTACGGCCAAGGCGT-3

′

Barcode-T-072, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGTGAATACCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-073, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCAATGACGTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-074, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAGGACGCTGACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-075, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTATCTGGGCCTGCTGTACGGCCAAGGCGT-3′

Barcoded adaptor sequence Length (nt)

1 2 ... 64 65 66 67 68 69 70 71 72 73 74 ... 86 87

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