Agilent Technologies E5500 Series Uživatelská příručka stáhnout pdf (Strana 68)

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixD Library Construction Oligonucleotide Sequences

Barcoded adaptor sequences

Barcode-T-052, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTAATTGTAACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-053, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTCATCAAGTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-054, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAAAGGCGGACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-055, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAGCTTAAGCGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-056, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCATGTCACCCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-057, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTCTAGTAAGAACTGCTGTACGGCCAAGGCGT-3′

Barcode-T-058, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTTAAAGTGGCGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-059, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGTAATGTCCTGCTGTACGGCCAAGGCGT-3

′

Barcode-T-060, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGTGCCTCGGTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-061, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAAGATTATCGCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-062, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTAGGTGAGGGTCTGCTGTACGGCCAAGGCGT-3′

Barcode-T-063, 50 µM

5′-CGCCTTGGCCGTACAGCAG-3′

5′-CTGCCCCGGGTTCCTCATTCTCTGCGGGTTCGACTGCTGTACGGCCAAGGCGT-3′

Barcoded adaptor sequence Length (nt)

1 2 ... 63 64 65 66 67 68 69 70 71 72 73 ... 86 87

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