Agilent Technologies DP235 Uživatelská příručka stáhnout pdf (Strana 157)

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Contents ▲ 157 ▼ Index

Principles of Flow Cytometric Measurements

Besides electrophoretic assays (DNA, RNA, and proteins), the Agilent 2100 bioanalyzer

supports flow cytometric assays:

• First, cells are stained with two fluorescent dyes that correspond to biologically

relevant parameters, as described in the application notes available for each assay.

“Staining Cells” on page 158 explains the principle.

• Next, the stained cells are analyzed on the chip. They pass the detector in single file and

are analyzed individually for their red and blue fluorescence intensities. The results are

displayed as histograms or dot plots. Refer to “Cell Detection with the Agilent 2100

Bioanalyzer” on page 160 for a detailed explanation.

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