Agilent Technologies DP235 Uživatelská příručka stáhnout pdf (Strana 237)

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Contents ▲ 237 ▼ Index

When using the calcein marker in the blue histogram for gating, only the living cells are

considered for building the red histogram. High red fluorescence values indicate living,

apoptotic cells, low red fluorescence values indicate living, non-apoptotic cells. See the

following example.

Low fluorescence value

indicates living non-apoptotic

cells

High fluorescence value

indicates living apoptotic cells

Percentage of all cells with

high red fluorescence

selected by the red marker

Amount of the living cells in relation to all measured cells

Amount of living cells with high red fluorescence in relation to the amount of living cells

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