Agilent Technologies DP235 Uživatelská příručka stáhnout pdf (Strana 199)

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Contents ▲ 199 ▼ Index

Using Histograms for Evaluation

Histograms are graphical representations of the measurement results, where the number

of events (cells) is mapped to the Y axis and their fluorescence values to the X axis. The

resulting curves show the frequency distribution of the events in relation to their

fluorescence intensity values, as shown in the following image. In real histograms, the

bin is reduced to a dot (data point).

The histograms can be evaluated statistically with markers that allow you to define

ranges of fluorescence intensity values. One histogram can be used to represent a range

of fluorescence values to define a subset of events. Only cells with a fluorescence value

within this range are displayed in the second histogram. This method is called gating.

50 cells were detected with a

fluorescence intensity between

30 and 40

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