
56 8000 Operator’s Manual
4 Operating the 8000
3 Prime Loss Volume Use this option to configure the prime loss volume. Prime loss volume
compensates for the volume of medium used to fill the tubing between the
sampling cannulas and sampling valves during the priming cycle. When sampling,
an amount of sample equal to the prime loss volume flows past the valves in the
8000 before the valves open to collect sample. The program also restricts this
volume to be less than or equal to the syringe size volume.
Select option 3, Prime Loss Volume. Enter a prime loss volume based on the
sample tubing length, inner diameter, and system dead volumes and press
ENTER. The Set Syringe Pump Parameters screen displays.
If the tubing length between the sampling cannulas and sampling valves is
changed, adjust the prime loss volume accordingly.
4 Aspirt. Dwell Time Use this option to configure the aspiration dwell time. Aspiration dwell time is the
time the syringe plunger pauses at the bottom of a stroke to allow the medium
being drawn to overcome the vacuum generated by the drawn plunger.
Select option 4, Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and
10 seconds and press ENTER The recommended time is 10 seconds. The Set
Syringe Pump Parameters screen displays.
5 Full Stroke (hidden
option)
Use this option to configure the number of steps the stepper motor makes to draw
the syringe plunger its maximum stroke length.
Select option 5, Full Stroke. Enter a full stroke value between 0 and 19999 and
press ENTER. The recommended value is 19300. The Set Syringe Pump
Parameters screen displays.
If the volume dispensed by the syringe is outside of the required specifications, the
full stroke value can be adjusted using the following calculation and solving for x:
target volume (mL)
= avg volume dispensed (mL)
x 19300
6 Pump Current (hidden
option)
Use this option to configure the pump current. The use of more viscous media
requires a higher pump current.
Select option 6, Pump Current. Enter a pump current between 0 and 8 and press
ENTER. The recommended value is 4. The Set Syringe Pump Parameters screen
displays.
Table 6 Set Syringe Pump Parameters (Apparatus 1 / 2)
Option Function
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