Agilent Technologies 8000 Series Návod k obsluze Strana 98

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98 8000 Operator’s Manual
4 Operating the 8000
5 The 8000 system monitor displays a reminder to ensure the tubing
and pump are ready. The Calibrate Sampling Volume screen
displays.
6 Select option 2, Automatic. The dispensing arm moves over the
autocalibration block and primes the lines according to the manual
prime settings. At the end of the priming cycle, all valves lower, open
simultaneously, and begin to fill the autocalibration block. As the 10
mL point is reached in each well, the valve closes and the time value
is recorded in memory. When the last valve closes, the pump reverses
and purges the lines. The calibration completion alarm, if enabled,
sounds when the calibration sequence is finished. Press any key to
silence the alarm.
7 After the dispensing arm moves forward, remove the autocalibration
block.
8 Clean and dry the separate portions of the autocalibration block.
9 Reconnect the top and bottom portions of the autocalibration block
and replace it.
10 Press ESC to return the dispensing arm to the home position.
***CALIBRATE SAMPLING VOLUME***
CHECK PUMP & TUBING. IF AUTO CALIBRATION
INSTALL CALIB FIXTURE BEFORE STARTING!
1 MANUAL 2 AUTOMATIC
NOTE
Because of intrinsic variations in tubing diameters, internal valve
passages, and pump tubing tensions, some valves must stay open longer
than others to deliver their nominal 10 mL calibration volumes. This is
normal operation.
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